Method of preparing polysaccharide possessing antitumor activity
专利摘要:
A fungus belonging to genus Coriolus of the class Basidiomycetes is subjected to submerged culture in a liquid medium and the obtained culture is dried and extracted with an aqueous solvent, and then the substances with molecular weights of less than 5,000 are eliminated from the extract solution, whereby a polysaccharide having anti-cancer and other pharmacodynamic effects is obtained in a high yield. 公开号:SU740156A3 申请号:SU772508258 申请日:1977-07-21 公开日:1980-06-05 发明作者:Есикуми Тикао;Вада Тосихико;Фудзии Масахико;Макита Хиромицу;Сузуки Кинзабуро;Синмио Акио;Хаяси Харухиса 申请人:Куреха Кагаку Когио Кабусики Кайся (Фирма); IPC主号:
专利说明:
In the case of submerged cultivation with the use of liquid medium, the micelles do not spread on the surface of the medium with the formation of pain iuHX masses, but are formed in the form of small ball-like or fibrous masses created by petemeshing. During this process, the polysaccharide is formed not only inside the migheli, but also in the connection itself. The nutrient medium is then dried, which is carried out at that temperature from 60 to. Drum gas-jet dryers and thin-film evaporators are used for drying; As a result of this treatment, extraction and water puff are simplified: Drill, as well as the collection of polysaccharide by means of purification of the extraction solution. After drying, the extraction is carried out with an aqueous solution containing an alkali, salt (sodium chloride, sodium acetate), pblngl organic solvent (for example, methanol, ethanol). Extraction is most effective with heating with water or a dilute alkali solution. The obtained extract solution is then processed to purify this solution by removing from it substances with a molecular weight less than 5000, which allows to obtain the target polysaccharide. Such treatment is carried out by salting out, dialysis, ultra ‑ eltraium, gel filtration or precipitation with an organic solvent. After removal of the low molecular weight components (with a molecular weight less than 5000) from the extract, the solution is spray dried or freeze dried followed by preparation of technical products. The polysaccharides obtained using the FP1 method are hepatic-copy-colored substances with a nitrogen content of from to%, the polysaccharides do not have a melting point, but gradually darken and decompose at a temperature exceeding about 120 ° C. Beirec is rattle in water, but it almost doesn’t fi alcohol, pyridine, chlorophenol, and hexane, it is almost tasteless and does not smell. Molecular weight ranges from 5,000 to 300,000. . Nitrogen-containing polysaccharide region gives a very strong antitumor effect, manifested in the treatment of mennane not only when inside the peritoneal injection into the body, but also when administered through the mouth. The proposed substance can be used not only to prevent undesirable side effects in cancer chemotherapy and sensitization to radiotherapy, but also to prevent susceptibility or the patient’s physical health following surgical procedures or blood transfusions, as well as to combat viral and bacterial diseases. . Example. 1600 l of nutrient medium containing 10% glucose, 1.5% yeast extract, 0.1% potassium phosphate and 0.1% magnesium sulfate heptahydrate are loaded into the fermentor, after which the nutrient medium is inoculated with 20 l of the fungal strain of CorJoIus versjcolor strain (FrJ Quel. IEEPM-P-Z414, obtained by shaking the culture, followed by cultivation for 7 days at a temperature of 26 ° C, aeration rate of 0.5 l / min / l of medium and rotational speed of the stirrer 150 rpm. Broth prepared in this way (sludge) is divided into 500-liter portions, after which each of these pans is dried in Double-drum type sinker. The surface temperature of the drum dryer is adjusted so that the surface temperature of the nutrient broth (the broth surface is determined by forming a thin layer of broth on the drum surface) is equal to 65 °, 80 °, 90 °, IO and 145C and the water content in each portion of the dried product is less than 20 wt.%. Then 250 l of hot water is added to each of these portions of the dried product and carried out - extraction treatment at a temperature of 95 ± 1 ° C for 3 hours, and after coolingmycelial residue Separated from extractor solution using a screw-type decanter and separated in a plate-type centrifugal separator. The polysaccharide products obtained are brown powder, readily soluble, in water. The polysaccharide obtained with the use of drying at a temperature of 60-150 has a high inhibitory activity. . The polysaccharide inhibits the growth of tumors. The following experiments were performed. Sarcoma-180 tumor cells are transplanted intraperitoneally into the mouse and, after 7 days of satisfactory growth, these cells are re-transplanted under: the skin of the axillary region of another mouse to produce solid tumors. The proposed substance is administered intraperitoneally in an amount of 10 mg / kg once a day for 20 days at a total dose of 0.2 ml / 20 g (mouse weight), and when administered orally, this substance gives an amount of 1000 mg / kg, day for 20 days with the introduction of a total amount of 0.2 ml / 20 g (weight of the pump). The nuclei of cells of all tumors die after 25 days after transplantation, the degree of tumor suppression is calculated based on the average weight of tumors in groups of patients who are given the proposed weight and average weight of tumors using control mice. The proposed method allows to obtain a drug with high antitumor activity. Thus, the magnitude of the activity of the preparation obtained by known and proposed methods is 100% and 95-98%, respectively, but the dose of the proposed preparation is 10 mg / kg, i.e., 60 times less than the dose of the known (600 mg / kg). The drug can be used in medicine in the treatment of malignant tumors.
权利要求:
Claims (1) [1] Claims method for producing a polysaccharide. It has an antitumor effect by submerged cultivation of the producer followed by extraction and purification of the target product, characterized in that, in order to increase anti-tumor activity, in quality. producer use a strain of Corlolus versicolor (Fr) Ouel. FERM-P-2414, Coriolus consors (Berk.) Amaz. FERM-p-988, Corjolas hlrsutus 5 (FrJ Quel. FERM-P-2711 or CorJol.us pargamenus (Ft.) Pat. FEBM-p-27i2 of class VazZbütusés and without extraction by extraction) the obtained culture liquid without separation of the mycelium is dried at a temperature of 60-150 0. Sources of information taken into account . 1. Japanese application 5G1-323 4, cl. 30 A 32, published. 1975.
类似技术:
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同族专利:
公开号 | 公开日 NL174568B|1984-02-01| AT360941B|1981-02-10| ATA531277A|1980-07-15| FR2359203A1|1978-02-17| PL103045B1|1979-05-31| IT1077413B|1985-05-04| DK334077A|1978-01-23| IN145992B|1979-02-03| HU175948B|1980-11-28| DE2731570A1|1978-01-26| RO72636B|1983-04-30| MX4285E|1982-03-12| BR7704806A|1978-03-28| RO72636A|1983-04-29| AU2700677A|1979-01-18| DD133444A5|1979-01-03| PH12983A|1979-10-29| GB1538399A|1979-01-17| CS222237B2|1983-05-27| NL174568C|1984-07-02| JPS5614276B2|1981-04-03| BG40486A3|1986-12-15| CA1088443A|1980-10-28| SE432614B|1984-04-09| PL199764A1|1978-03-13| US4140578A|1979-02-20| NL7707614A|1978-01-24| FR2359203B1|1980-04-18| ZA773934B|1978-05-30| ES460885A1|1978-05-01| DE2731570C3|1980-03-27| SE7708401L|1978-01-23| BE856805A|1978-01-16| YU39808B|1985-04-30| YU163177A|1982-08-31| JPS5315495A|1978-02-13| DK144890B|1982-06-28| CH631485A5|1982-08-13| DK144890C|1982-11-22| AU514350B2|1981-02-05| AR220680A1|1980-11-28| DE2731570B2|1979-07-26|
引用文献:
公开号 | 申请日 | 公开日 | 申请人 | 专利标题 US3301848A|1962-10-30|1967-01-31|Pillsbury Co|Polysaccharides and methods for production thereof| US3759896A|1968-03-28|1973-09-18|T Yamamoto|Process for manufacture of polysaccharides with antitumor action| JPS4843841B1|1969-09-06|1973-12-21| BE757248A|1969-10-15|1971-04-08|Kureha Chemical Ind Co Ltd|SUBSTANCE WITH ANTI-CANCER PROPERTY AND METHODS FOR ITS PREPARATION| US4051314A|1970-10-14|1977-09-27|Kureha Kagaku Kogyo Kabushiki Kaisha|Polysaccharides and method for producing same| JPS4924211A|1972-07-01|1974-03-04| JPS57793B2|1972-09-16|1982-01-07| JPS5628152B2|1975-12-18|1981-06-30| JPS5614275B2|1976-07-07|1981-04-03| JPS5614274B2|1976-07-07|1981-04-03|US4851395A|1976-07-07|1989-07-25|Kureha Kagaku Kogyo Kabushiki Kausha|Nitrogen-containing polysaccharide| JPS5614274B2|1976-07-07|1981-04-03| JPS5646481B2|1977-01-27|1981-11-04| IT1158896B|1977-08-10|1987-02-25|Hepar Chimie Sa|HEXURONYL HEXOSAMINOGLICAN NATURAL SULPHATE, PROCEDURE FOR ITS PREPARATION AND RELATED THERAPEUTIC USES| JPS5726645B2|1977-09-16|1982-06-05| JPS5461112A|1977-10-24|1979-05-17|Ono Pharmaceut Co Ltd|Oncostatic polysaccharide its preparation and oncostatic drugs containing it as an effective component| US4268505A|1978-04-13|1981-05-19|Kureha Kagaku Kogyo Kabushiki Kaisha|Pharmaceutical composition comprising a nitrogen-containing polysaccharide and an antibiotic agent, and a method of treating an infectious disease therewith| IT1141263B|1980-02-29|1986-10-01|Italfarmaco Spa|METHOD FOR THE PREPARATION OF CALCIUM HEPARINATE| JPS5932480B2|1981-02-10|1984-08-09|Kureha Chemical Ind Co Ltd| US4614733A|1981-12-31|1986-09-30|Kureha Kagaku Kogyo Kabushiki Kaisha|Polysaccharides pharmaceutical compositions and the use thereof| DE3448155C2|1983-08-11|1989-11-02|Kureha Kagaku Kogyo K.K., Nihonbashi, Tokio/Tokyo, Jp| WO1988006627A1|1987-02-26|1988-09-07|Bio Polymers Pty. Ltd.|Plant gum material and use thereof in food products| US4861432A|1987-11-03|1989-08-29|Huyck Corporation|Dual compartment vacufoil unit to eliminate secondary headbox chatter| US7048932B2|2002-05-22|2006-05-23|The Chinese University Of Hong Kong|Preparation and standardization of immunomodulatory peptide-linked glucans with verifiable oral absorbability from coriolus versicolor| US7829098B2|2005-11-08|2010-11-09|Vita Green Health Products Co., Ltd|Herbal powder extracts and methods of preparing and using the same| US7854936B2|2008-01-14|2010-12-21|Active Organics, Inc.|Anti-inflammatory hydrolysate of C. versicolor |
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申请号 | 申请日 | 专利标题 JP8750376A|JPS5614276B2|1976-07-22|1976-07-22| 相关专利
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